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Analysis of intraspecies polymorphism in the ribosomal DNA cluster of the cockroach Blattella germanica
Author(s) -
Mukha D. V.,
Sidorenko A. P.,
Lazebnaya I. V.,
Wiegmann B. M.,
Schal C.
Publication year - 2000
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2000.00175.x
Subject(s) - biology , hindiii , genetics , ribosomal dna , restriction fragment length polymorphism , ribosomal rna , population , cockroach , german cockroach , spacer dna , dna , gene , genotype , restriction enzyme , phylogenetics , ecology , demography , sociology
Hin dIII restriction digests of the rDNA repeat unit of the German cockroach, Blattella germanica , reveal significant intraspecies sequence polymorphism. This variability is probably caused by structural differences within the nontranscribed spacer regions (NTS) of the ribosomal repeat unit. Hin dIII rDNA fragment polymorphisms in three cockroach strains show that individuals from different populations may have different Hin dIII rDNA patterns, whereas individuals within populations exhibit relatively similar rDNA patterns. We suggest that Hin dIII restriction fragment polymorphisms within cockroach ribosomal DNA will be a valuable tool for measuring population‐level parameters within and between natural cockroach populations.