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Characterization of a carboxypeptidase A gene from the mosquito, Aedes aegypti
Author(s) -
Edwards M. J.,
Moskalyk L. A.,
DonellyDoman M.,
Vlaskova M.,
Noriega F. G.,
Walker V. K.,
JacobsLorena M.
Publication year - 2000
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2000.00159.x
Subject(s) - aedes aegypti , biology , anopheles gambiae , midgut , carboxypeptidase , messenger rna , microbiology and biotechnology , gene , open reading frame , carboxypeptidase a , gene expression , aedes , in situ hybridization , genetics , peptide sequence , biochemistry , larva , enzyme , immunology , botany , malaria
A gut‐specific carboxypeptidase A gene ( AeCPA ) from the mosquito, Aedes aegypti , was cloned and characterized. The gene has an open reading frame that predicts a protein of 427 amino acids, 61% of which are identical to an Anopheles gambiae carboxypeptidase A sequence. AeCPA messenger RNA (mRNA) was not detected during larval and pupal development. In situ hybridization experiments indicated that AeCPA mRNA is expressed by posterior midgut epithelial cells. In sharp contrast to An. gambiae carboxypeptidase A gene expression, AeCPA mRNA accumulates to high levels only late (≈ 16–24 h) after ingestion of a blood meal. The temporal profile of AeCPA gene induction is similar to that of Ae. aegypti late trypsin, suggesting the existence of common regulatory elements.