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Cloning and characterization of the ribosomal protein CcP0 of the medfly Ceratitis capitata
Author(s) -
Gagou M.E.,
Ballesta J. P. G.,
Kouyanou S.
Publication year - 2000
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.2000.00156.x
Subject(s) - biology , ribosomal protein , eukaryotic large ribosomal subunit , ceratitis capitata , eukaryotic small ribosomal subunit , ribosome , ribosomal rna , eukaryotic ribosome , complementary dna , genetics , microbiology and biotechnology , gene , biochemistry , 18s ribosomal rna , botany , rna , pest analysis , tephritidae
The gene of the ribosomal protein CcP0, the third member of the ribosomal P‐protein family of the medfly Ceratitis capitata , was identified by genomic and cDNA sequence analysis. It codes for a polypeptide of 317 amino acids and its predicted amino acid sequence shows great similarity to the P0 proteins of other eukaryotic organisms. The CcP0 gene was expressed in Escherichia coli and the 34‐kDa recombinant protein was identical to the P0 protein of purified medfly ribosomes. Both proteins reacted positively with a specific monoclonal antibody against the highly conserved C terminus of eukaryotic ribosomal P proteins. Interestingly, the medfly CcP0 seems to be the only P0 protein of higher eukaryotic organisms with basic character (pI 8.5), as shown by electrofocusing of purified ribosomes.