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Proteins from nuclear extracts of two lepidopteran cell lines recognize the ends of TTAA‐specific transposons piggyBac and tagalong
Author(s) -
Field L. M.,
James A. A.,
Bauser C. A.,
Elick T. A.,
Fraser M. J.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.820223.x
Subject(s) - transposable element , biology , retrotransposon , exonuclease iii , genetics , dna , microbiology and biotechnology , gene , genome , escherichia coli
The transposons piggyBac and tagalong are Lepidopteran transposons that exhibit extreme site‐specificity for the tetranucleotide TTAA upon insertion and excise in a characteristic precise fashion, regenerating a single TTAA target site. The precise excision of both piggyBac and tagalong can occur in the absence of factors encoded by either transposon, possibly through the recruitment of host proteins and/or cross‐mobilizing transposons. In this report, we utilize mobility shift assays and exonuclease III protection analyses to identify DNA binding activities from IPLB‐SF21AE and TN‐368 cells that are specific for piggyBac and tagalong ‐terminal repeats.