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Genetic similarity among pheromone and voltinism races of Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae)
Author(s) -
Field L. M.,
James A. A.,
Marçon P. C. R. G.,
Taylor D. B.,
Mason C. E.,
Hellmich R. L.,
Siegfried B. D.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.820213.x
Subject(s) - ostrinia , european corn borer , crambidae , biology , lepidoptera genitalia , voltinism , restriction fragment length polymorphism , genetic variation , pyralidae , botany , gene flow , genetics , polymerase chain reaction , gene , larva
The genetic variability of seven European corn borer populations, Ostrinia nubilalis , from North America and Europe was assessed by polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) analysis and DNA sequencing. The nuclear ribosomal internal transcribed spacer 1 (ITS‐1) region (≈ 500 base pair [bp]) and four mitochondrial (mtDNA) regions (1550 bp total) were examined. The smartweed borer, Ostrinia obumbratalis , and south‐Western corn borer, Diatraea grandiosella , were used for comparisons. Of 106 restriction sites identified (80 in mtDNA and 26 in ITS‐1), none differentiated geographical populations, pheromone races, or voltine ecotypes of the European corn borer. The lack of variation in the ITS‐1 of European corn borer was confirmed by DNA sequence analysis. The genetic similarity of European corn borer populations, despite their wide geographical range and physiological differences, may be explained by a relatively recent origin for the voltinism and pheromone races, gene flow among races, and/or expansion from genetic bottlenecks.