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Typing of sandflies from Greece and Cyprus by DNA polymorphism of 18S rRNA gene
Author(s) -
Field L. M.,
James A. A.,
Aransay A. M.,
Scoulica E.,
Chaniotis B.,
Tselentis Y.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.820179.x
Subject(s) - biology , restriction fragment length polymorphism , 18s ribosomal rna , typing , genetics , gene , polymorphism (computer science) , evolutionary biology , ribosomal rna , polymerase chain reaction , genotype
A simple and reliable technique was developed to distinguish Phlebotomine sandflies by restriction fragment length polymorphism of PCR‐amplified (PCR‐RFLP) 18S rDNAs. Seven morphologically identified sandflies species from several localities of Greece and Cyprus were studied, and specific patterns were developed by double digesting amplified 18S rDNAs with Hpa ll and Rsa l. Three additional species of the sub‐genus Larroussius were distinguished by a second double digestion with Acc l and Ban l. We have successfully applied the method on samples in which morphological characters were badly distinguished due to poor storage conditions and in larval stages.