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Voltage‐gated sodium channel genes hscp and hDSC1 of Heliothis virescens F. genomic organization
Author(s) -
Field L. M.,
James A. A.,
Park Y.,
Taylor M. F. J.,
Feyereisen R.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.820161.x
Subject(s) - biology , exon , gene , genetics , drosophila melanogaster , coding region , alternative splicing , genomic dna , homology (biology) , microbiology and biotechnology , rna splicing , locus (genetics) , scn3a , g alpha subunit , protein subunit , rna
We report the genomic sequence of hscp , a sodium channel α subunit gene for Heliothis virescens . A 32‐kb genomic clone and six independent RT‐PCR products covering almost the entire coding region of the gene, contained thirty‐one deduced exons with a translation of 1695 residues. Overall amino acid similarity to the para locus of Drosophila melanogaster was 86%. The transcription of the gene was complex. Alternate splicing was evident for five optional exons and a pair of mutually exclusive exons. A number of alternatively spliced mRNA revealed a deduced translation product that included only the first homology domain. We also report the first partial sequence for hDSC1 , a presumed orthologous of the DSC1 sodium channel α subunit gene of D. melanogaster .