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Precise excision and transposition of piggyBac in pink bollworm embryos
Author(s) -
Thibault S. T.,
Luu H. T.,
Vann N.,
Miller T. A.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.810119.x
Subject(s) - biology , transposable element , transposition (logic) , drosophila melanogaster , transformation (genetics) , p element , plasmid , genetics , pink bollworm , aedes aegypti , gene , botany , genome , larva , linguistics , philosophy
Transposable elements such as P, hobo, Hermes, mariner and Minos have been successfully harnessed as gene vectors to achieve the transformation of several dipteran species including Drosophila melanogaster, Ceratitis capitata and Aedes aegypti . Plasmid‐based excision and transposition assays have been useful indicators of an element's ability to be mobilized in vivo and thus potentially serve as a transforming vector. We report that the transposable element piggyBac is capable of precise excision and transposition in the pink bolloworm ( Pectinophora gossypiella ), a worldwide pest of cultivated cotton. Combined with a suitble marker gene, the piggyBac element may serve as a vector for germline transformation in this and (potentially) other lepidopteran species.