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Characterization of the soluble guanylyl cyclase β‐subunit gene in the mosquito Anopheles gambiae
Author(s) -
Caccone A.,
Garcia B. A.,
Mathiopoulos K. D.,
Min G.S.,
Moriyama E. N.,
Powell J. R.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.810023.x
Subject(s) - anopheles gambiae , biology , protein subunit , gene , guanylate cyclase , soluble guanylyl cyclase , rna interference , midgut , microbiology and biotechnology , genetics , botany , malaria , receptor , immunology , larva , rna
Genomic DNA corresponding ot the soluble guanylyl cyclase β ‐subunit (GCS β ) gene was cloned and sequenced form Anopheles gambiae . The sequence was 8103 bp long and presumably included the entire coding region. The deduced amino acid sequence was 71% and 62% similar to previously known Drosophila and vertebrate GCS β , while the C‐terminus of A. gambiae GCS β was shorter. Because of the conserved characteristics in each functional domain, the high G+C % in the third codon positions compared to the introns, the lack of internal stop codons, and the fact that we identified the gene from a cDNA, we conclude that this A. gambiae gene is functional. This is the first detailed description of a guanylyl cyclase gene structure (e.g. intron–exon boundaries). Interstingly, within the fifth intron we found high similarity to the flanking regions of the Pegasus‐27 transposable element and other noncoding regions of the A. gambiae genome.