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Cloning and analysis of cDNAs encoding the hypusine‐containing protein eIF5A of two lepidopteran insect species
Author(s) -
Van Oers Monique M.,
Van Marwijk Mireille,
Kwa Marcel S. G.,
Vlak Just M.,
Thomas Adri A. M.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.00148.x
Subject(s) - spodoptera , biology , complementary dna , exigua , gene , drosophila melanogaster , cloning (programming) , genetics , cdna library , peptide sequence , microbiology and biotechnology , recombinant dna , computer science , programming language
Eukaryotic initiation factor eIF5A is essential for cell viability and contains a characteristic post‐translational modification of a specific lysine residue into a hypusine. cDNAs with similarity to eIF5A sequences were derived from Spodoptera exigua and S. frugiperda cDNA libraries. The deduced amino acid sequences are identical for both species and predict a protein with a molecular mass of 17.5 kDa. The Drosophila melanogaster eIF5A cDNA sequence was retrieved from the Drosophila EST Project. The predicted protein is 80% similar to Spodoptera eIF5A. A single eIF5A gene copy is present in the S. frugiperda genome, which is transcribed into four different transcripts. Infection of S. frugiperda cells with a baculovirus resulted in a strong decline of all four transcripts already at 12 h after infection. In contrast, the eIF5A protein was fairly stable up to 48 h post infection.