Premium
Relative abundance, isolation and structure of phlebotomine microsatellites
Author(s) -
Day J. C.,
Ready P. D.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.00142.x
Subject(s) - biology , microsatellite , isolation (microbiology) , evolutionary biology , abundance (ecology) , zoology , computational biology , ecology , genetics , gene , bioinformatics , allele
Popular classes of microsatellites are not always abundant in insects or easily isolated from them. Dot‐blot hybridizations demonstrated much variation in the relative abundance of four repeat classes in four phlebotomine sandfly species. Only AAT‐class repeats were specifically isolated from a phagemid library of Lutzomyia whitmani , even though other microsatellites had similar abundances. An enrichment step would have targeted classes but was omitted because relatively long flanking sequences were sought. All fourteen sandfly loci had a non‐coding structure, and a minority of dipteran AAT‐class repeats found in DNA databases and the literature were from exons. Therefore, this class should often provide neutral alleles for population studies. Perfect, not imperfect, AAT‐class repeats were polymorphic in wild L. whitmani.