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Sequence of a mosquito ribonucleotide reductase cDNA and evidence for gene amplification in hydroxyurea‐resistant cells
Author(s) -
Shotkoski F. A.,
Jayachandran G.,
Stamatoyannopoulos G.,
Fallon A. M.
Publication year - 1999
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1999.00136.x
Subject(s) - ribonucleotide reductase , biology , complementary dna , gene , microbiology and biotechnology , aedes albopictus , nucleic acid sequence , cdna library , amino acid , protein subunit , biochemistry , botany , aedes aegypti , larva
Ribonucleotide reductase is essential for production of the deoxyribonucleotide substrates required for DNA synthesis in all eukaryotic cells. The full‐length cDNA encoding a mosquito ribonucleotide reductase R2 subunit was obtained from Aedes albopictus cells using a polymerase chain reaction (PCR)‐based approach. The cDNA contained 1197 nucleotides, and encoded a 398 amino acid R2 protein. Overall, mosquito R2 shared approximately 70% amino acid sequence identity with R2 proteins from vertebrates, but conservation at the N‐terminus of the protein was relatively low. The sequence of R2 cDNA was identical in hydroxyurea‐resistant mosquito cells and in wild‐type cells, while the R2 gene copy number was increased ten to twentyfold in resistant cells.