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Two‐dimensional gel analysis of midgut proteins of Anopheles stephensi lines with different susceptibility to Plasmodium falciparum infection
Author(s) -
Prévot G. I.,
LaurentWinter C.,
Feldmann A. M.,
Rodhain F.,
Bourgouin C.
Publication year - 1998
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1998.740375.x
Subject(s) - midgut , anopheles stephensi , biology , plasmodium falciparum , malaria , parasite hosting , anopheles , virology , immunology , aedes aegypti , botany , larva , world wide web , computer science
Little is known about the composition of the mosquito midgut which plays a central role in the development and subsequent transmission of malaria parasites. As a first step towards the characterization of mosquito midgut molecules involved in the transmission of malaria parasites, we analysed two‐dimensional gel electrophoresis patterns of the midgut proteins of sugar‐fed and blood‐fed Anopheles stephensi lines of different susceptibility to P. falciparum infection. Two lines fully susceptible and one line (Pb3‐9A) of reduced susceptibility were used. In the refractory line ookinetes do develop but are only inefficiently transformed into oocysts (Feldmann & Ponnudurai, 1989). The protein profiles of midguts from all sugar‐fed mosquito lines were similar. However, after blood feeding, the midgut of the fully susceptible lines contained proteins not found in the midgut of line Pb3‐9A. Twenty‐nine such proteins were detected and are candidates for involvement in the interaction between the mosquito midgut and P. falciparum .