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An estimate of phylogenetic relationships among culicine mosquitoes using a restriction map of the rDNA cistron
Author(s) -
Kumar A.,
Black W. C.,
Rai K. S.
Publication year - 1998
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1998.740367.x
Subject(s) - biology , cistron , phylogenetic tree , restriction fragment length polymorphism , restriction enzyme , restriction site , maximum parsimony , monophyly , phylogenetics , ecorv , taxon , genetics , evolutionary biology , zoology , clade , botany , hindiii , gene , polymerase chain reaction , escherichia coli
Restriction maps of the rDNA cistron of twelve species of mosquitoes in six genera of the subfamily Culicinae were constructed using eight 6 bp recognition restriction enzymes. Anopheles albimanus was used as an outgroup. The size of the rDNA cistron ranged from 8.5 kb in Aedes katherinensis to 12.9 kb in Ae. polynesiensis . A total of twenty‐six sites were scored; eighteen were polymorphic among ingroup taxa. The proportion of polymorphic nucleotide sites (Pnuc) was 0.059 and the heterozygosity per nucleotide site (Hnuc) was 0.028. Wagner and Fitch Parsimony, Dollo Parsimony and Nei‐Li distance/neighbour‐joining methods were used to construct phylogenetic trees. The rDNA RFLP dataset did not provide a well‐supported phylogeny among culicine taxa. The RFLP phylogenies are incongruent with the morphology character based and molecular phylogenies and derived relationships did not correspond with current taxonomic classifications. The lack of resolution was due to homoplasy arising from frequent independent loss or gain of restriction sites among unrelated taxa.