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Molecular cloning and nucleotide sequenceof a cytochrome P450 cDNA from a pyrethroid‐resistant mosquito, Culex quinquefasciatus Say
Author(s) -
Kasai S.,
Shono T.,
Yamakawa M.
Publication year - 1998
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1998.72053.x
Subject(s) - biology , culex quinquefasciatus , complementary dna , cdna library , nucleic acid sequence , microbiology and biotechnology , clone (java method) , sequence analysis , genetics , permethrin , gene , larva , botany , aedes aegypti , pesticide , agronomy
A novel cDNA clone encoding a cytochrome P450 was screened from a gut cDNA library of permethrin‐resistant Culex quinquefasciatus larvae. Nucleotide sequence of the clone designated CYP6E1 was determined. This is the first full‐length sequence of a mosquito P450 cDNA. Comparison of the deduced amino acid sequence of CYP6E1 to those of other known cytochrome P450s indicates that this clone belongs to CYP6 family. In addition, a phylogenetic analysis of CYP6E1 primary structure showed that it is related to CYP6A and CYP6C subfamilies. Polymerase chain reaction (PCR) using primers designed on the basis of the nucleotide sequence of the cDNA showed that mRNA encoding CYP6E1 is present in permethrin‐susceptible larvae as well as ‐resistant larvae, suggesting the ubiquitous distribution of CYP6E1.