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A member of the hsp70 gene familyfrom the Mediterranean fruit fly, Ceratitis capitata
Author(s) -
Thanaphum S.,
Haymer D. S.
Publication year - 1998
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1998.71051.x
Subject(s) - ceratitis capitata , biology , capitata , botany , gene , mediterranean climate , tephritidae , horticulture , genetics , pest analysis , ecology , brassica oleracea
A gene from the Mediterranean fruit fly (Medfly) Ceratitis capitata , belonging to the hsp70 family of genes, has been cloned and characterized. This gene appears to be an example of a heat shock cognate (hsc) gene based on a variety of structural and functional criteria. The heat shock cognate genes serve critical roles in chaperoning of cellular proteins, and they are expressed in a more constitutive manner compared to the inducible heat shock genes. We determined the sequence of the entire coding region of this gene (designated Cerhsc1) together with 5′ and 3′ flanking regions. The coding region exhibits specific structural features that are characteristic of the subclass of heat shock genes producing proteins localized to the cytosol. The 5′ regulatory region of Cerhsc1 contains only the minimal array of canonical heat shock element sequences, a phenomenon commonly seen in heat shock cognates. Abundant transcripts are detected from this gene in adult tissue compared to the larval stage. In larval tissue, heat shock appears to produce only a slight increase in the level of transcripts detected. Based on overall structure and function, this Medfly gene appears to be an ortholog of heat shock cognate gene 4 (Drohsc4) of Drosophila melanogaster.