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Molecular cloning and chromosomal localization of a prophenoloxidase cDNA from the malaria vector Anopheles gambiae
Author(s) -
Lee W.J.,
Ahmed A.,
Della Torre A.,
Kobayashi A.,
Ashida M.,
Brey P. T.
Publication year - 1998
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1998.71047.x
Subject(s) - anopheles gambiae , prophenoloxidase , biology , cloning (programming) , complementary dna , vector (molecular biology) , anopheles , molecular cloning , genetics , malaria , gene , virology , microbiology and biotechnology , computational biology , immunology , recombinant dna , immune system , innate immune system , computer science , programming language
A cDNA clone for prophenoloxidase was isolated from the most important human malaria vector, Anopheles gambiae . The clone encoded a polypeptide of 79341 Da that contains the two copper binding domains common to all invertebrate prophenoloxidases and haemocyanins. Expression of the prophenoloxidase gene was detected throughout all life stages from egg to imago in two strains of A. gambiae ; however, the strongest expression was observed in developing embryos in eggs. The prophenoloxidase gene was mapped to the inversion rich region of the right arm of chromosome‐2 in region 13B.