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The white gene of the tephritid fruit fly Bactrocera tryoni is characterized by a long untranslated 5′ leader and a 12 kb first intron
Author(s) -
Bennett C. L.,
Frommer M.
Publication year - 1997
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1997.00188.x
Subject(s) - biology , exon , genetics , intron , genomic dna , gene , white (mutation) , microbiology and biotechnology , exon trapping , complementary dna , alternative splicing
A 300 bp fragment from exon 6 of the white gene of Bactrocera tryoni was used to screen a B. tryoni genomic library. One positive (∼14 kb) insert contained exons 2–6 of white by nucleotide and amino acid sequence similarity to the white genes of D. melanogaster (O'Hare et al ., 1984; Pepling & Mount, 1990). Lucilia cuprina (Garcia et al ., 1996). Ceratitis capitata (Zwiebel et al ., 1995) and Anopheles gambiae (Besansky et al ., 1995). A white 5′ cDNA fragment containing exons 1, 2 and part of exon 3 was amplified, cloned and sequenced. An inverse PCR fragment of genomic DNA was generated, containing the exon 1 coding region plus ∼2.1 kb of upstream sequence, encompassing the putative promoter of the gene. Exon 1 was found to be 728 bp long, encoding the first twenty‐five amino acids. The full length of intron 1 was shown to be 12 kb (amplified using long PCR protocols), up to 3 times the length of the longest white intron 1 isolated to date.