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Cloning and sequence of a gene for a homologue of the C subunit of the V‐ATPase from the salivary gland of the tick Amblyomma americanum (L.)
Author(s) -
McSwain J. L.,
Luo C.,
DeSilva G. A.,
Palmer M. J.,
Tucker J. S.,
Sauer J. R.,
Essenberg R. C.
Publication year - 1997
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1997.00158.x
Subject(s) - amblyomma americanum , biology , microbiology and biotechnology , complementary dna , salivary gland , atpase , saliva , clone (java method) , cloning (programming) , ouabain , gene , tick , virology , enzyme , biochemistry , ixodidae , sodium , chemistry , organic chemistry , computer science , programming language
A 1084 base pair partial cDNA showing similarity to the C subunit of the vacuolar ATPase (V‐ATPase) was isolated on a clone from a cDNA library made from salivary glands from 3‐day‐old feeding adultAmblyomma americanum(L.) female ticks. The 5′ end was completed using primer extension and the two pieces joined to form a complete cDNA of 1373 bp. This mRNA is expressed in embryos and the salivary glands of unfed adults and adult females at all stages of feeding. Specific inhibitors of the V‐ATPase decrease the rate of dopamine‐stimulated secretion of isolated salivary glands, but not as much as ouabain, an inhibitor of the Na + , K + ATPase, indicating that a V‐ATPase may participate in the mechanism of salivary fluid secretion inA. americanum, but the volume of saliva secreted is more dependent on an active Na + , K + ATPase.