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Analysis of eriophyid mite rDNA internal transcribed spacer sequences reveals variable simple sequence repeats
Author(s) -
Fenton B.,
Malloch G.,
Moxey E.
Publication year - 1997
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1046/j.1365-2583.1997.00152.x
Subject(s) - biology , internal transcribed spacer , indel , ribosomal dna , genetics , mite , spacer dna , ribosomal rna , sequence analysis , botany , dna , gene , phylogenetics , genotype , single nucleotide polymorphism
Ribosomal DNA internal transcribed spacers of the eriophyid mitesCecidophyopsis ribis,C. selachodon,C. spicata,C. alpina,C. aurea,C. grossulariaeandPhylocoptes gracilliswere amplified using PCR, cloned and sequenced. Sequences for the ITS1 ofCecidophyopsidswere 92–99% homologous.Cecidophyopsisinter‐specific differences were found in seventeen simple sequence repeats (vSSRs), fourteen point mutations and two indels. No intra‐specific variation in vSSRs was detected. A hypothetical structure for ITS1 was obtained and vSSRs were mapped onto this. Changes in vSSRs were compensated for by changes in complementary vSSRs or through multiple point mutations. A comparison with vSSRs of other arthropods suggested that the levels of intra‐specific variation inCecidophyopsismites was less than in organisms which do not use arrhentoky for male determination.