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In vivo expression of interleukin‐8, and regulated on activation, normal, T‐cell expressed, and secreted, by human germinal centre B lymphocytes
Author(s) -
SimsMourtada Jennifer C.,
GuzmanRojas Liliana,
Rangel Roberto,
Nghiem Dat X.,
Ullrich Stephen E.,
Guret Christiane,
Cain Kelly,
MartinezValdez Hector
Publication year - 2003
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2003.01745.x
Subject(s) - germinal center , chemokine , biology , lymphocyte homing receptor , homing (biology) , b cell , t cell , microbiology and biotechnology , cxc chemokine receptors , cxcl16 , cxcr5 , receptor , immunology , t lymphocyte , cc chemokine receptors , cxcl14 , chemokine receptor , cell , antigen , immune system , antibody , cell adhesion , ecology , biochemistry , genetics
Summary T‐cell homing within germinal centres (GCs) is required for humoral B‐cell responses. However, the mechanisms implicated in the recruitment of T cells into the GC are not completely understood. Here we show, by immunohistology, and Northern and Western blots, that in vivo human GC B lymphocytes can express CxC and CC chemokines. Moreover, B‐cell subset‐specific experiments reveal that interleukin (IL)‐8 and regulated on activation, normal, T‐cell expressed, and secreted (RANTES) are predominantly expressed by GC centroblast and centrocytes, suggesting that chemokine expression is essential at stages in which B‐lymphocytes engage in active antigen‐dependent interactions with T lymphocytes. In keeping with this hypothesis, we show that the T cells recruited into the GC correlatively express the receptors for IL‐8 and RANTES. We propose that chemokine expression is a key B‐cell function that facilitates T‐lymphocyte recruitment into the GCs and supports cognate B‐cell : T‐cell encounters. Moreover, our data are consistent with the impaired homing of T cells to secondary lymphoid organs in mice that are either deficient in CC and CxC chemokines or their receptors.