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Genomic structure around joining segments and constant regions of swine T‐cell receptor α/δ ( TRA/TRD ) locus
Author(s) -
Uenishi Hirohide,
Hiraiwa Hideki,
Yamamoto Ryuji,
Yasue Hiroshi,
Takagaki Yohtaroh,
Shiina Takashi,
Kikkawa Eri,
Inoko Hidetoshi,
Awata Takashi
Publication year - 2003
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2003.01695.x
Subject(s) - biology , conserved sequence , enhancer , genetics , locus (genetics) , sequence (biology) , nucleic acid sequence , regulatory sequence , gene , peptide sequence , regulation of gene expression , transcription factor
Summary A complete genomic region of 131·2 kb including the swine T‐cell receptor α/δ constant region ( TRAC / TRDC ) and joining segments ( TRAJ / TRDJ ) was sequenced. The structure of this region was strikingly conserved in comparison to that of human or mouse. All of the 61 TRAJ segments detected in the human genomic sequence were detected in the swine sequence and the sequence of the protein binding site of T early alpha, the sequence of the α enhancer element and the conserved sequence block between TRAJ3 and TRAJ4 are highly conserved. Insertion of the repetitive sequences that interspersed after the differentiation of the species in mammals such as short interspersed nucleotide elements is markedly suppressed in comparison to other genomic regions, while the composition of the mammalian‐wide interspersed sequences is relatively conserved in human and swine. This observation indicates the existence of a highly selective pressure to conserve this genomic region around TRAJ throughout the evolution of mammals.