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Phenotypic profile and functional characterization of rat lymph node‐derived γδ T cells: implication in the immune response to cytomegalovirus
Author(s) -
Dyugovskaya Larissa,
Hirsh Mark,
Ginsburg Haim
Publication year - 2003
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2003.01568.x
Subject(s) - immune system , biology , immunology , fibroblast , in vitro , lymph , cytomegalovirus , lymph node , virology , microbiology and biotechnology , virus , herpesviridae , pathology , medicine , viral disease , biochemistry
Summary γδ T cells are unique, and their localization at sites of infection is considered critical in immune defence. We demonstrate the accumulation of γδ T cells in rat regional popliteal lymph nodes (PLNi) starting 2 days after inoculation of cytomegalovirus (CMV) into the footpad. Early‐appearance PLNi γδ T cells significantly inhibited plaque development and the spread of CMV infection . These γδ T cells were negative for CD4 and CD8beta receptors , proliferated in response to interleukin‐2 (IL‐2) and contained high levels of interferon‐γ (IFN‐γ), the appearance of which correlated with the curing of fibroblasts from virus infection. The addition of anti‐IFN‐γ abolished the ability of fibroblast monolayers to be cured from CMV infection. In contrast, this protection was not abolished by the addition of anti‐rat IL‐2 or anti‐rat TNF‐α, or by the depletion of NKR‐P1‐bearing cells within γδ T cells. In addition, the present study shows that while γδ T cells derived from naive and CMV‐infected rats are able to kill both YAC‐1 targets and CMV‐infected syngeneic fibroblasts in vitro , only the latter are able to clear CMV‐infected fibroblast monolayers. Finally, our data suggest that the expression of NKR‐P1 by γδ T cells is critical for cytotoxicity, but its contribution to the curing from CMV infection was limited.