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Tumour necrosis factor‐α but not lipopolysaccharide enhances preference of murine dendritic cells for Th2 differentiation
Author(s) -
Kikuchi Kazuhiro,
Yanagawa Yoshiki,
Aranami Toshimasa,
Iwabuchi Chikako,
Iwabuchi Kazuya,
Onoé Kazunori
Publication year - 2003
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2003.01537.x
Subject(s) - lipopolysaccharide , ovalbumin , tumor necrosis factor alpha , antigen , immunology , dendritic cell , biology , chemistry , microbiology and biotechnology
Summary Using murine spleen‐derived dendritic cells (DC) and DO11.10 T cells specific for ovalbumin (OVA), the influences of maturational condition and antigen dose on the capability of DC to induce helper T‐cell (Th) differentiation were analysed. Immature DC (iDC) with high‐ or low‐dose OVA 323–339 predominantly induced Th1 or Th2 responses in DO11.10 T cells, respectively. DC matured by tumour necrosis factor‐α (TNF/DC) induced a significantly higher Th2 response in the presence of low‐dose OVA 323–339 than iDC and DC matured by lipopolysaccharide (LPS) (LPS/DC). In the presence of high‐dose OVA 323–339 , LPS/DC induced significantly lower levels of Th1 response than iDC. Under these conditions no difference in the Th1 response was noted between TNF/DC and iDC. The enhanced capability of TNF/DC with a low‐dose antigen for Th2 polarization and the decreased preference of LPS/DC with a high‐dose antigen to Th1 polarization were not related to the amount of IL‐12 produced in these cultures. These results demonstrate for the first time that TNF/DC with a low‐dose antigen are potent inducers of Th2 differentiation.