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Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes
Author(s) -
Chan Simon S. M.,
Mcconnell Ian,
Blacklaws Barbara A.
Publication year - 2002
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2002.01515.x
Subject(s) - cd14 , cd11c , immunology , biology , peripheral blood mononuclear cell , mhc class ii , dendritic cell , antigen presenting cell , major histocompatibility complex , monocyte , antigen presentation , microbiology and biotechnology , in vitro , antigen , immune system , t cell , phenotype , gene , biochemistry
Summary Dendritic cells (DCs) are professional antigen‐presenting cells with a highly immunostimulatory function and the capacity to activate naïve T cells. In recent years the rapid progress in mouse and human DC research can be mainly attributed to the generation of DCs from precursor cells in vitro , although a lack of reagents has hampered DC research in many large animal models. Here we describe the generation and characterization of ovine monocyte‐derived DCs in vitro . In addition to the characteristic morphology and non‐adherence of DCs, peripheral blood mononuclear cell monocytes cultured with ovine granulocyte–macrophage colony‐stimulating factor (GM–CSF) and interleukin‐4 (IL‐4) expressed CD11c and major histocompatibility complex (MHC) class II, but did not express CD14. High levels of endocytosis and an ability to stimulate antigen‐specific proliferation of CD4 T lymphocytes were also demonstrated.