Premium
Cross‐presentation of cell‐associated antigens by CD8α + dendritic cells is attributable to their ability to internalize dead cells
Author(s) -
Schulz Oliver,
Reis e Sousa Caetano
Publication year - 2002
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2002.01513.x
Subject(s) - cross presentation , antigen presentation , microbiology and biotechnology , cd8 , dendritic cell , mhc class i , antigen , biology , t cell , antigen presenting cell , immunology , immune system
Summary In the mouse, cross‐presentation is an exclusive property of the CD8α + subset of dendritic cells (DC) but the basis for this selectivity remains unclear. Here we report that splenic CD8α + DC are much superior to other DC subsets in internalizing dying cells in vitro . In contrast, CD8α + , CD8α – CD4 + and CD8α – CD4 – DC subsets phagocytose bacteria or latex beads to a similar extent. Although CD8α + DC are better than CD4 + DC at presenting ovalbumin (OVA)‐loaded splenocytes to naïve OT‐I T lymphocytes, CD4 + DC are better at presenting OVA‐expressing Escherichia coli to the same T cells. In both cases, presentation is abrogated by lactacystin. These results show that both splenic CD8α + and CD8α – DC can present exogenous antigens on major histocompatibility complex (MHC) class I via a proteasome‐dependent pathway and suggest that the specialized cross‐presenting function of CD8α + DC is a result of their ability to endocytose dying cells rather than a unique pathway for handling endosomal contents.