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Maternal cell traffic bounds for immune modulation: tracking maternal H‐2 alleles in spleens of baby mice by DNA fingerprinting
Author(s) -
Wan Wenhan,
Shimizu Shoji,
Ikawa Hiromichi,
Sugiyama Kiyosh,
Yamaguchi Nobuo
Publication year - 2002
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2002.01499.x
Subject(s) - biology , antigen , placenta , allele , major histocompatibility complex , offspring , immune system , immunology , antibody , fetus , polymerase chain reaction , microbiology and biotechnology , gene , genetics , pregnancy
Summary We have previously reported that the immunization of pregnant mice with T‐dependent antigens successfully induced suppression of the antigen‐specific plaque‐forming cell (PFC) response to the relevant antigens in the offspring. This suppression was not caused by the administered antigens, the antibodies produced by the pregnant mother, or lactational transfer, but was dependent on the presence of the intact maternal T cells. It was major histocompatibility complex (MHC)‐restricted manner tolerance, which continued for at least one‐sixth of the murine life. Traditionally, the placenta acts as a natural barrier, not allowing the cells to pass through. However, the results presented strongly suggested that maternal T cells pass through the placenta and subsequently induce tolerance. In this present study, we attempted to substantiate the presence of maternal cells in the fetal circulation through the use of molecular techniques. We found that a highly polymorphic microsatellite sequence within the class II Eb gene of the H‐2 complex is useful for the molecular detection of various H‐2 alleles. DNA polymorphic analysis was used for tracking maternal H‐2 alleles in the spleens of baby mice. The main procedure involved polymerase chain reaction amplification and restriction fragment length polymorphism analysis of the DNA sequence encompassing the H‐2‐specific microsatellite from the genomic DNA of baby mice. The results indicated that maternal T cells of immunized pregnant mice cross the placenta into the fetus, eventually inducing antigen‐specific immunological tolerance in the offspring.