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Involvement of tumour necrosis factor‐α‐related apoptosis‐inducing ligand in enhanced cytotoxicity of lipopolysaccharide‐stimulated dendritic cells to activated T cells
Author(s) -
Yu Yizhi,
Liu Shuxun,
Wang Wenya,
Song Wengang,
Zhang Minghui,
Zhang Weiping,
Qin Zhihai,
Cao Xuetao
Publication year - 2002
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2002.01431.x
Subject(s) - cytotoxic t cell , apoptosis , tumor necrosis factor alpha , microbiology and biotechnology , immune system , lipopolysaccharide , programmed cell death , dendritic cell , antigen presenting cell , t cell , biology , cd40 , chemistry , cancer research , immunology , biochemistry , in vitro
Summary Dendritic cells (DC) are potent antigen‐presenting cells (APC) specialized in T‐cell mediated immune responses, and also play critical roles in the homeostasis of T cells for controlling immune responses. In the present study, we demonstrated that during mouse bone‐marrow‐derived DC activation of ovalbumin (OVA)‐specific Ia‐k b ‐restricted T hybridoma cells, MF2.2D9 and OVA 257–264 ‐specific H‐2k b ‐restricted RF33.70 T cells, respectively, both hybridomas undergo cell death, partially mediated via apoptotic ligand–tumour necrosis factor‐α (TNF‐α)‐related apoptosis‐inducing ligand (TRAIL). Lipopolysaccharide enhanced the cytotoxic effect on the two activated T hybridoma cells, which was correlated with up‐regulation of TRAIL‐expression on DC to some extent. The activation of caspase‐3 in activated T hybridoma cells cocultured with DC contributed to the programmed cell death pathway T cells underwent. Therefore, our results show that activation‐induced cell death of T hybridoma cells can be influenced by DC, suggesting that DC may be involved in elimination of activated T cells at the end of primary immune responses.