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Lipopolysaccharide triggers invasive streptococcal disease in mice through a tumour necrosis factor‐α‐dependent mechanism
Author(s) -
Diao Hongyan,
Kohanawa Masashi,
Nakajima Hirofumi,
Sato Yuichiro,
Minagawa Tomonori,
Nakane Akio
Publication year - 2002
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2002.01372.x
Subject(s) - tumor necrosis factor alpha , necrosis , streptococcus pyogenes , lipopolysaccharide , biology , toxic shock syndrome , infiltration (hvac) , microbiology and biotechnology , monoclonal antibody , antibody , immunology , bacteria , staphylococcus aureus , genetics , physics , thermodynamics
Summary Streptococcus pyogenes sometimes induces invasive streptococcal infection, including streptococcal toxic shock syndrome (STSS). Muscular necrosis is one of the peculiar symptoms of invasive streptococcal infection and STSS. We inoculated S. pyogenes into the muscles of mice. To do so, 5 × 10 8 bacteria in 0·2 ml phosphate‐buffered saline were injected into the right hind thigh. None of the mice injected with the bacteria showed muscular necrosis and none died. Tumour necrosis factor‐α (TNF‐α) and infiltration of leucocytes were detected in the muscles of infected sites, although the condition of the infected mice did not deteriorate after anti‐TNF‐α monoclonal antibody treatment. The infected mice treated intraperitoneally with Escherichia coli lipopolysaccharide (LPS) showed augmentation of bacterial growth, muscular necrosis and death. TNF‐α was detected in the sera of the infected mice treated with LPS, but not in the muscles of the infected sites. Infiltration of leucocytes into the infected muscle was not observed in the infected mice treated with LPS. Anti‐TNF‐α monoclonal antibody treatment decreased mortality in the infected mice treated with LPS. Moreover, the infected mice treated with recombinant TNF‐α showed augmentation of muscular necrosis and death. These results suggest that systemic production of TNF‐α induced by stimulation with LPS inhibits infiltration of leucocytes into the infected site and exacerbates muscular infection, and that TNF‐α produced in streptococcal infection is not a defence factor for the host. Invasive streptococcal infection and STSS appear to be induced by both S. pyogenes and the host's immune system.