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Failure to induce enhanced protection against tuberculosis by increasing T‐cell‐dependent interferon‐γ generation
Author(s) -
Leal Irene S.,
Smedegård Birgitte,
Andersen Peter,
Appelberg Rui
Publication year - 2001
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2001.01305.x
Subject(s) - mycobacterium tuberculosis , tuberculosis , adjuvant , monoclonal antibody , interferon gamma , immunology , recombinant dna , virology , antibody , neutralization , cytokine , biology , medicine , microbiology and biotechnology , pathology , biochemistry , gene
Summary We evaluated the use of recombinant human interleukin‐6 (rhIL‐6) and a monoclonal antibody specific for interferon‐γ (IFN‐γ) as co‐adjuvants in a subunit vaccine against tuberculosis consisting of the culture filtrate proteins of Mycobacterium tuberculosis (ST‐CF) emulsified in the adjuvant dimethyl‐dioctadecylammonium bromide (DDA). Both the addition of rhIL‐6 and the neutralization of IFN‐γ resulted in an increased T helper type 1 (Th1) response characterized by enhanced IFN‐γ production and cell proliferation. Nevertheless, this did not result in the enhancement of protection against either an intravenous or an aerosol M. tuberculosis challenge. Our data stress the need to identify further correlates of protection in addition to IFN‐γ production to screen vaccines against tuberculosis infection.