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Porcine dendritic cells generated in vitro : morphological, phenotypic and functional properties
Author(s) -
Carrasco Carlos P.,
Rigden Rachael C.,
Schaffner Rene,
Gerber Heidi,
Neuhaus Viviane,
Inumaru Shigeki,
Takamatsu Haru,
Bertoni Guiseppe,
McCullough Kenneth C.,
Summerfield Artur
Publication year - 2001
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2001.01299.x
Subject(s) - cd14 , cd80 , dendritic cell , biology , antigen presentation , mhc class ii , microbiology and biotechnology , monocyte , t cell , immunology , major histocompatibility complex , antigen presenting cell , immune system , cytotoxic t cell , cd40 , in vitro , biochemistry
Summary Despite the central role that dendritic cells (DC) play in immune regulation and antigen presentation, little is known about porcine DC. In this study, two sources of DC were employed. Bone marrow haematopoietic cell‐derived DC (BM‐DC) were generated using granulocyte–macrophage colony‐stimulating factor (GM‐CSF) in the presence or absence of tumour necrosis factor‐α (TNF‐α). Monocyte‐derived DC (Mο‐DC) were generated with GM‐CSF and interleukin‐4 (IL‐4). In both systems, non‐adherent cells developed with dendritic morphology, expressing high levels of major histocompatibility complex (MHC) class II. The presence of TNF‐α increased the BM‐DC yield, and enhanced T‐cell stimulatory capacity. Both BM‐DC and Mο‐DC expressed the pan‐myeloid marker SWC3, as well as CD1 and CD80/86, but were also CD14 + and CD16 + . The CD16 molecule was functional, acting as a low‐affinity Fc receptor. In contrast, the CD14 on DC appeared to differ functionally from monocyte CD14: attempts to block CD14, in terms of lipopolysaccharide (LPS)‐induced procoagulant activity (PCA), failed. The use of TNF‐α or LPS for DC maturation induced up‐regulation of MHC class II and/or CD80/86, but also CD14. Allogeneic mixed leucocyte reactions and staphylococcal enterotoxin B antigen presentation assays demonstrated that these DC possessed potent T‐cell stimulatory capacity. No T helper cell polarization was noted. Both the BM‐DC and the Mο‐DC induced a strong interferon‐γ and IL‐4 response. Taken together, porcine DC generated in vitro possess certain characteristics relating them to DC from other species including humans, but the continued presence of CD14 and CD16 on mature and immature porcine DC was a notable difference.