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In vitro stimulation with a non‐peptidic alkylphosphate expands cells expressing Vγ2‐Jγ1.2/Vδ2 T‐cell receptors
Author(s) -
Evans Peter S.,
Enders Patrick J.,
Yin Cheng,
Ruckwardt Tracy J.,
Malkovsky Marek,
Pauza C. David
Publication year - 2001
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2001.01282.x
Subject(s) - t cell receptor , biology , major histocompatibility complex , t cell , stimulation , receptor , immunology , population , polyclonal antibodies , antigen , microbiology and biotechnology , immune system , genetics , endocrinology , medicine , environmental health
Summary The majority of peripheral blood γδ T cells in healthy adult humans express the Vγ2/Vδ2 T‐cell receptor (TCR) and generate TCR‐mediated, major histocompatibility complex (MHC)‐unrestricted proliferative responses to low molecular weight alkylphosphates. Vγ2/Vδ2 populations after antigen proliferation maintained diversity in the CDR3s of Vγ2 mRNA, indicating that the response was polyclonal or oligoclonal, and were enriched for Vγ2 TCR chains containing the Jγ1.2 segment. Alkylphosphate stimulation further skewed an already biased peripheral blood γδ T‐cell population and increased the abundance of Vγ2‐Jγ1.2/Vδ2 T cell receptors, suggesting similarities between the alkylphosphate response and peripheral selection mechanisms shaping this repertoire in human beings.