Intraspecies heterogeneity of immunoglobulin α‐chain constant region genes in rhesus macaques

Summary Immunoglobulin A (IgA) is the major antibody class present in external secretions and is also an important component of serum immunoglobulins. On mucosal surfaces, IgA represents a first line of defence by neutralizing invading pathogens. The number of IgA constant‐region genes (Cα) present in different mammalian species is variable. Immunoglobulin Cα genes differ mainly in the sequences located in the hinge region. IgA molecules, whose hinge regions are remarkably similar to those of the respective human molecules, are present in hominoid primates. In this report, we show that two alleles of a single immunoglobulin Cα are present in rhesus macaques ( Macaca mulatta ). In addition, we show that intraspecies immunoglobulin Cα allelic polymorphism is very high in this non‐human primate species. Specifically, five different hinge regions, some of which are proline‐rich, were identified from a total of eight rhesus macaque immunoglobulin Cα‐chains. The five hinge regions were different from those present in hominoid primates, both in length and in sequence. These results represent the first example of high levels of intraspecies immunoglobulin constant‐region variability and suggest that IgAs of variable structure and function may be present in rhesus macaques. As rhesus macaques are widely used as animal models for the development of vaccines for acquired immune deficiency syndrome (AIDS), the possible presence of structurally and functionally variable IgA molecules in different animals should be taken into account when designing experimental strategies to induce mucosal antibody responses to human immunodeficiency virus (HIV).