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Human B‐cell lines constitutively express and secrete interleukin‐16
Author(s) -
Sharma V.,
Sparks J. L.,
Vail J. D.
Publication year - 2000
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2000.00959.x
Subject(s) - secretion , biology , reverse transcriptase , cd8 , microbiology and biotechnology , immune system , messenger rna , cell culture , b cell , transcription (linguistics) , reverse transcription polymerase chain reaction , polymerase chain reaction , virology , immunology , gene , antibody , endocrinology , genetics , linguistics , philosophy
Summary Interleukin‐16 (IL‐16), produced by activated CD8 + T lymphocytes, is inhibitory to human immunodeficiency virus‐1 (HIV‐1) replication. In an attempt to determine whether human B cells express and secrete IL‐16, a wide panel of B‐cell lines derived from patients with acquired immune deficiency syndrome (AIDS)‐associated B‐cell lymphomas (AABCL) ( n = 5) and from non‐AABCLs ( n = 8) were studied. Using reverse transcription–polymerase chain reaction (RT–PCR) analysis, we were able to observe ubiquitous expression of IL‐16 mRNA. Kinetic studies on constitutive mRNA turnover and secretion for IL‐16 suggests that the optimum expression is at 24 hr. Interestingly, we report, for the first time, IL‐16 secretion by human B‐cell lines.