Premium
Hydrogen peroxide augments eosinophil adhesion via β 2 integrin
Author(s) -
Nagata M.,
Yamamoto H.,
Shibasaki M.,
Sakamoto Y.,
Matsuo H.
Publication year - 2000
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2000.00123.x
Subject(s) - cd18 , integrin alpha m , integrin , adhesion , cd11a , eosinophil , cell adhesion molecule , chemistry , cell adhesion , microbiology and biotechnology , tumor necrosis factor alpha , immunology , biochemistry , biology , cell , organic chemistry , asthma
Summary During eosinophil (EOS) accumulation at sites of allergic inflammation, an initial step is the binding of EOS to adhesion molecules expressed on vascular endothelial cells (EC). We have previously observed that adhesion of peripheral blood EOS to recombinant human vascular cell adhesion molecule‐1 (rh‐VCAM‐1) stimulates the respiratory burst of EOS. Although the biological consequence of this activation remains to be elucidated, reactive oxygen species such as hydrogen peroxide (H 2 O 2 ) may modify the adhesive property of EOS. In the present study, we examined whether H 2 O 2 modifies the adhesive property of EOS. EOS were isolated from the peripheral blood of healthy subjects. Adhesion of the EOS to paraformaldehyde‐fixed human umbilical vein EC (HUVEC), stimulated or not stimulated with tumour necrosis factor‐α (TNF‐α; 100 p m for 24 hr), was examined in the presence or absence of H 2 O 2 . H 2 O 2 significantly enhanced adhesion of EOS to both resting and TNF‐α‐stimulated fixed HUVEC ( P < 0·01, respectively). Such enhancing effects were inhibited by anti‐β 2 integrin antibody or anti‐CD11b antibody, but not by anti‐CD11a or anti‐α 4 integrin antibody. H 2 O 2 also enhanced EOS adhesion to rh‐intracellular cell adhesion molecule‐1 (ICAM‐1) but not to rh‐VCAM‐1. Finally, H 2 O 2 enhanced the expression of both CD11b and CD18 on EOS. These results indicate that H 2 O 2 directly augments the adhesive property of EOS through β 2 integrin.