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The contribution of both oxygen and nitrogen intermediates to the intracellular killing mechanisms of C1q‐opsonized Listeria monocytogenes by the macrophage‐like IC‐21 cell line
Author(s) -
ÁlvarezDomínguez C.,
CarrascoMarín E.,
LópezMato P.,
LeyvaCobián F.
Publication year - 2000
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2000.00083.x
Subject(s) - listeria monocytogenes , opsonin , intracellular , macrophage , microbiology and biotechnology , oxygen , listeria , biology , nitrogen , chemistry , phagocytosis , bacteria , biochemistry , genetics , in vitro , organic chemistry
Summary Listeria monocytogenes is a facultative intracellular pathogen which is internalized by host mammalian cells upon binding to their surface. Further listerial growth occurs in the cytosol after escape from the phagosomal–endosomal compartment. We have previously reported that C1q is able to potentiate L. monocytogenes phagocytosis upon bacterial opsonization by ingestion through C1q‐binding structures. In this report, we analysed the post‐phagocytic events upon internalization of C1q‐opsonized L. monocytogenes and found an induction of macrophage (Mφ)‐like IC‐21 cell bactericidal mechanisms displayed by the production of oxygen and nitrogen metabolites. Both types of molecules are effective in L. monocytogenes killing. Further analysis of the cellular responses promoted by interaction of C1q with its surface binding structures, leads us to consider C1q as a collaborative molecule involved in Mφ activation. Upon interaction with surface binding structures, C1q was able to trigger and/or amplify the production of reactive oxygen and nitrogen intermediates induced by stimuli such as interferon‐γ and L. monocytogenes phagocytosis.