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Activation of T‐cell receptor‐γδ + cells in the intestinal epithelia of KN6 transgenic mice
Author(s) -
KawaguchiMiyashita M.,
Shimada S.,
Matsuoka Y.,
Ohwaki M.,
Nanno M.
Publication year - 2000
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2000.00076.x
Subject(s) - intraepithelial lymphocyte , t cell receptor , microbiology and biotechnology , cd8 , biology , cd44 , antigen , t cell , t lymphocyte , chemistry , immunology , immune system , cell , biochemistry
Summary We analysed the properties of intraepithelial lymphocytes of small intestine (SI‐IEL) in KN6‐transgenic (Tg) mice expressing cDNA of T‐cell receptor (TCR)‐γδ specific for the T22 b molecule. While most splenic Tg TCR‐γδ + cells from KN6‐Tg mice with H‐2 d/d background (Tg d/d mice) were Thy‐1 + CD8α – CD44 dull+ CD45RB + CD69 – , Tg TCR‐γδ + cells in SI‐IEL (Tg γδ‐IEL) were heterogeneous in the expression of Thy‐1, CD8α and CD44 molecules and predominantly CD45RB + CD69 + . Tg γδ‐IEL exhibited a much reduced proliferative response to the antigen (irradiated H‐2 b splenocytes) than splenic Tg TCR‐γδ + cells; the CD44 + subset, but not the CD44 – subset, in Tg γδ‐IEL responded to the antigen. Furthermore, Tg γδ‐IEL, but not splenic Tg TCR‐γδ + cells, displayed cytolytic activity whether they were prepared from conventional or germ‐free KN6‐Tg mice. Comparative analysis of young and aged KN6‐Tg mice revealed that the proportion of CD44 + cells in Tg γδ‐IEL increased but the proliferative response of Tg γδ‐IEL to the antigen attenuated in association with ageing. Moreover, although Tg γδ‐IEL from Tg b/d mice contained a higher proportion of CD44 + cells than Tg d/d mice, they did not respond to the antigen. These results demonstrate that Tg TCR‐γδ + cells lose the ability to recognize the antigen following activation in the intestinal epithelia.