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Dimerization of major histocompatibility complex class I on the surface of THP‐1 cells stimulates the expression of inducible nitric oxide synthase and subsequent nitric oxide release
Author(s) -
Bottley G.,
Fernández N.
Publication year - 2000
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.2000.00060.x
Subject(s) - thp1 cell line , major histocompatibility complex , nitric oxide synthase , nitric oxide , microbiology and biotechnology , monoclonal antibody , flow cytometry , mhc class i , biology , innate immune system , antibody , mhc class ii , cell culture , messenger rna , chemistry , immune system , immunology , biochemistry , gene , genetics , endocrinology
Summary We show that dimerization of major histocompatibility complex (MHC) class I on a human monocytic cell line, THP‐1, induces nitric oxide (NO) synthesis. Cells cultured in the presence of a human MHC class I‐specific monoclonal antibody produced significant amounts of NO after 72 hr. Reverse transcription–polymerase chain reaction and flow cytometry analysis revealed that the cells synthesized detectable levels of inducible NO synthase mRNA and protein. These effects were not seen after treatment with monovalent Fab fragments or Fc fragments of the same antibody, or after treatment with a control antibody. These data show a link between innate and acquired immune mechanisms mediated by NO and MHC class I.