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Systematic characterization of porcine ileal Peyer’s patch, II. A role for CD154 on T cells in the positive selection of immature porcine ileal Peyer’s patch B cells
Author(s) -
Andersen J. K.,
Takamatsu H.,
Pullen L.,
Parkhouse R. M. E.
Publication year - 1999
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1999.00923.x
Subject(s) - cd154 , biology , cd40 , microbiology and biotechnology , peyer's patch , b cell , follicular phase , immunology , antibody , antigen , cytotoxic t cell , in vitro , endocrinology , biochemistry
Summary We previously demonstrated that the majority (≥ 90%) of porcine ileal Peyer’s patch (IPP) follicular cells are immature B cells destined to die by apoptosis, when incubated at 37°. In this paper we approached the mechanisms responsible for positive selection of porcine IPP follicular immature B‐cell selection, by screening for various cell types, cytokines and polyclonal and monoclonal antibodies for promoting the survival of IPP B cells. Of these reagents, only CD3 cross‐linked purified T cells from mesenteric lymph nodes were able to rescue IPP follicular B cells from apoptosis, although polyclonal anti‐IPP lymphocyte antibodies delayed apoptosis. This survival effect could be reproduced simply by incubating IPP follicular B cells with soluble and cell membrane‐expressed CD154, an observation consistent with the demonstrated presence of CD40 and CD154 on porcine IPP follicular B cells and activated T cells, respectively. The IPP follicular B cells rescued in this manner expressed a more mature surface marker phenotype. Immunohistology and fluorescence‐activated cell sorter analysis demonstrated that subpopulations of IPP follicular T cells (less than 0·5%) express CD154. Thus, perhaps unexpectedly, CD154 on T cells may play a role in the positive selection of immature B cells in the porcine IPP. The origin and control of the activated T cells identified within the porcine IPP remains to be investigated.