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The expansion of human γδ T cells in response to Daudi cells requires the participation of CD4 + T cells
Author(s) -
Fayen,
Tykocinski
Publication year - 1999
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1999.00761.x
Subject(s) - monoclonal antibody , t cell , cd8 , major histocompatibility complex , human leukocyte antigen , epitope , biology , antigen , t lymphocyte , cytotoxic t cell , immune system , immunology , cell culture , microbiology and biotechnology , antibody , in vitro , biochemistry , genetics
The Burkitt’s lymphoma cell line Daudi is a potent inducer of human γδ T‐cell expansion. Using an in vitro culture system comprised of irradiated Daudi cells as stimulators and normal human lymphocytes as responders, the cellular determinants of this response were investigated. Three of four monoclonal antibodies (mAbs 1‐1C4, L243, and 9.3F10) directed against disparate epitopes of human major histocompatibility complex (MHC) class II, as well as a mAb with specificity for CD4 (OKT4), inhibited the expansion of γδ T cells in response to Daudi cell stimulators. mAbs with a specificity for CD74 and CD8 were non‐inhibitory. Lymphocyte depletion experiments demonstrated a critical role for the CD4 + T‐cell subset in the expansion of γδ T cells. Other data pointed towards requirements for direct cell contact in this system, and the addition of exogenous recombinant interleukin (IL)‐2, IL‐4, and IL‐12 failed to reconstitute γδ T‐cell expansion in CD4 + lymphocyte‐depleted cultures. These results complement previous findings in murine infectious disease and mycobacterial systems, providing a direct demonstration that CD4 + T cells play a role in γδ T‐cell expansion through an interaction with human leucocyte antigen (HLA) class II on Daudi cells. The data point towards important functional links between the acquired and natural immune systems.