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Enhancement of B7‐1 (CD80) expression on B‐lymphoma cells by irradiation
Author(s) -
Akira Seo,
Fumio Ishikawa,
Hideki Nakano,
Hisao Nakazaki,
Kenzo Kobayashi,
Terutaka Kakiuchi
Publication year - 1999
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1999.00720.x
Subject(s) - cd80 , microbiology and biotechnology , antigen , cd40 , biology , chemistry , in vitro , immunology , cytotoxic t cell , biochemistry
Irradiation of A20.2J mouse B‐lymphoma cells enhanced their antigen‐presenting ability to induce interleukin‐2 (IL‐2) production by 42‐6A T cells specific for ovalbumin (OVA) 323–339 /I‐A d . Irradiated and fixed A20.2J cells were more efficient antigen‐presenting cells (APC) to present OVA 323–339 peptide than the unirradiated and fixed cells. Irradiation selectively increased the expression of B7‐1 molecules, but not of the major histocompatibility complex class II molecules, B7‐2, lymphocyte function‐associated antigen‐1, or intracellular adhesion molecule‐1. Irradiation of A20.2J cells with 100 Gy followed by overnight incubation was optimal for the enhancement of B7‐1 expression. Anti‐B7‐1 monoclonal antibody inhibited the irradiation‐induced enhancement of APC function. Irradiation of A20.2J cells induced the accumulation of B7‐1 mRNA. Thus, it was concluded that the enhancement of APC function by irradiation was due to the up‐regulation of B7‐1 molecules through the accumulation of its mRNA. Although partial inhibition of protein synthesis has been shown to enhance the accumulation of B7‐1 mRNA and its expression, irradiation did not decrease the protein synthesis in A20.2J cells. The incubation with irradiated A20.2J cells stimulated unirradiated A20.2J cells to increase B7‐1 expression, suggesting that irradiation of A20.2J cells induced expression or secretion of some molecule(s) to enhance B7‐1 expression.