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B‐cell antigens within normal and activated human T cells
Author(s) -
G. P. Sandilands,
Mark Perry,
Martha Wootton,
James Hair,
I. A. R. More
Publication year - 1999
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1999.00692.x
Subject(s) - antigen , pan t antigens , cytoplasm , biology , microbiology and biotechnology , immunoelectron microscopy , flow cytometry , staining , endoplasmic reticulum , cell , antigen presenting cell , t cell , in vitro , antibody , immunology , cytotoxic t cell , immune system , biochemistry , genetics
In this study we compared cell surface staining for human peripheral blood lymphocyte (PBL) CD antigens by flow cytometry, with staining obtained following permeabilization of PBL using the Cytoperm method (Serotec). Six CD antigens (CD20, CD21, CD22, CD32, CD35 and major histocompatibility complex class II antigen) normally found on the surface of B cells, were also found to be expressed within T cells. We also showed, by immunoelectron microscopy, that these inappropriately expressed (‘occult’) CD antigens are located within cytoplasmic vesicles or within the rough endoplasmic reticulum. Following in vitro activation of T cells a distinct increase in expression of all of these cytoplasmic antigens was observed but staining at the cell surface was, by comparison, weak. We therefore propose that up‐regulation of various B‐cell CD antigens occurs within the cytoplasm of T cells following activation and that these antigens may be synthesized and released into the fluid‐phase as soluble immunoregulatory molecules.