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U5 monoclonal antibody identifies a novel lymphocyte surface antigen preferentially expressed in human circulating natural killer cells with high cytotoxic activity
Author(s) -
Ikura,
Terao,
Matsuzaki,
InoueMurayama,
Murayama
Publication year - 1999
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1999.00687.x
Subject(s) - cd16 , cytotoxic t cell , biology , granzyme b , antigen , microbiology and biotechnology , perforin , k562 cells , granzyme , lymphokine activated killer cell , natural killer cell , granzyme a , monoclonal antibody , cd49b , nk 92 , interleukin 21 , antibody , cd3 , immunology , cd8 , leukemia , in vitro , biochemistry
U5 monoclonal antibody (mAb), developed against Japanese monkey lymphocytes, identified a glycoprotein of 32 000 MW that is expressed in a subset of human circulating natural killer (NK) cells. The distribution of U5 molecules was restricted among CD16 + cells, and U5 antigen was preferentially expressed in the CD38 + subset. U5 + CD16 + CD56 + cells were highly active on NK assay against K562 target cells. Variations in cytolytic activities and mRNA expression of perforin, granzyme B and Fas ligand (FasL) were observed in U5 − CD16 + CD56 + cells depending on the donor. We found that in some donors, a phenotypically mature (CD16 + CD56 + ) but functionally immature subset was present in the peripheral circulation. The U5 − CD16 + CD56 + cells of some donors exhibited negligible cytolytic activity with no detectable expression of the above mRNAs, whereas those of the other donors had a significant but lower cytolytic activity with a reduced expression of granzyme B mRNA as compared with those of U5 + CD16 + CD56 + cells. Concanavalin A (Con A) stimulation induced an expression of U5 antigen in U5 − CD16 + CD56 + cells accompanied by an up‐regulation of granzyme B mRNA expression. These findings suggest that U5 antigen may be a novel molecule involved in the maturation or differentiation of human circulating NK cells.