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Characterization of immune responses following intramuscular DNA immunization with the MOMP gene of Chlamydia trachomatis mouse pneumonitis strain
Author(s) -
D J Zhang,
Xi Yang,
Caixia Shen,
Robert C. Brunham
Publication year - 1999
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1999.00682.x
Subject(s) - immune system , biology , chlamydia trachomatis , immunization , immunology , antibody , dna vaccination , humoral immunity , virology , antigen , chlamydiaceae , cellular immunity , microbiology and biotechnology
Studies were carried out to characterize the cellular and humoral immune responses evoked by intramuscular DNA vaccination with the major outer membrane protein (MOMP) gene of Chlamydia trachomatis mouse pneumonitis strain. The data demonstrate that DNA vaccinated mice develop antigen‐specific delayed‐type hypersensitivity, lymphocyte proliferation and interferon‐γ (IFN‐γ) production. Serum antibody responses (mainly immunoglobulin G2a; IgG2a) were evoked in two‐thirds of the mice. We conclude that intramuscular DNA immunization with the MOMP gene evokes cellular and humoral immune responses suggestive of a T helper 1 (Th1) bias.