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Lymphocyte‐specific protein 1: a specific marker of human leucocytes
Author(s) -
Karen Pulford,
Margaret Jones,
Alison H. Banham,
E Haralambieva,
David Y. Mason
Publication year - 1999
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1999.00677.x
Subject(s) - biology , epitope , lymphocyte , lymphoma , cd19 , anaplastic large cell lymphoma , monoclonal antibody , microbiology and biotechnology , antibody , immunology
While both murine and human homologues of the LSP1 gene (lymphocyte‐specific gene 1) and its protein products have been identified, studies on human LSP1 have been limited. The present report describes a detailed immunocytochemical study of the distribution and localization of human LSP1 in both normal and neoplastic cells and tissues. The specificity of the monoclonal anti‐LSP1 reagent was confirmed by expression cloning and transfection studies. The intracellular 60 000 MW LSP1 protein was found to be present in peripheral blood B cells, monocytes and granulocytes but absent in a subpopulation of circulating T cells (10–15% of CD3‐positive T cells). The presence of LSP1 protein in medullary thymocytes, but only in scattered cortical thymocytes, provided additional evidence for heterogeneity of expression in T cells. Novel observations also included the presence of LSP1 in plasma cells, dendritic cells and Langerhans’ cells. The leucocyte‐restricted distribution of LSP1 protein means that it may play an important role in haematopathology. LSP1 protein was detected in a wide range of leukaemias and lymphomas, particularly of B‐cell origin, and in tumour cells in classical Hodgkin’s disease. Of interest was the indication of a reciprocal relationship in the expression of LSP1 and ALK (anaplastic lymphoma kinase) proteins in patients with anaplastic large cell lymphoma. As the anti‐LSP1 reagent used in the present study recognizes a formalin‐resistant epitope it should be of considerable value in the diagnosis of routinely fixed material.