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B‐1‐like cells exist in sheep. Characterization of their phenotype and behaviour
Author(s) -
Nathalie Chevallier,
Madeleine Berthelemy,
Véronique Lainé,
Danielle Le Rhun,
Françoise Féménia,
Bruno Polack,
Jan Naessens,
Daniel Lévy,
Isabelle Schwartz-Cornil
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00599.x
Subject(s) - phenotype , biology , cd5 , b cell , antibody , cd11c , naive b cell , microbiology and biotechnology , b 1 cell , immunology , immune system , t cell , genetics , antigen presenting cell , gene
Two populations of B lymphocytes, B‐1 (CD5 + and/or CD11b + ) and B‐2 (CD5 − and CD11b − ) cells have been described. In mice, which is the species of reference for B‐1 and B‐2 cell studies, these two subsets present different developmental schemes, phenotypes, antibody repertoires, localization and behaviours. Interestingly, in sheep, B cells rearrange their immunoglobulin (Ig) loci around the neonatal period, similarly to murine B‐1 cells. However, the phenotype of the sheep B cells has not been characterized with regards to their developmental pathway. In this report, we show that two sheep B‐cell subsets can be distinguished on the basis of CD11b expression. Relative to CD11b − B cells, the CD11b + B cells frequently co‐express CD5, CD11c, higher levels of surface IgM (sIgM), show larger cell size and higher cell‐cycling activity, and thus present a B‐1‐like phenotype. However, unlike murine B‐1 cells, sheep B‐1 like cells mainly localize in blood, display a higher propensity to spontaneous apoptosis relative to B‐2‐like cells, and proliferate after sIgM stimulation. Our data show that despite neonatal immunoglobulin loci rearrangements, sheep B cells do not all express a B‐1‐like phenotype. However, B‐1‐and B‐2‐like cells co‐exist and present phenotypic and behavioural specificities. Nevertheless, sheep B‐1‐and B‐2‐like cells differ from the murine B‐1 and B‐2 cells in their cell behaviour. These subsets can thus not be considered as true homologues among species.