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Tissue distribution of the guinea‐pig decay‐accelerating factor
Author(s) -
Kazuhiro Nishikawa,
Seiichi Matsuo,
Hiroaki Tamai,
Noriko Okada,
Hidechika Okada
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00592.x
Subject(s) - guinea pig , biology , staining , microbiology and biotechnology , monoclonal antibody , immunofluorescence , epithelium , antibody , immunology , endocrinology , genetics
MCA44 is a monoclonal antibody (mAb) to guinea‐pig decay‐accelerating factor (DAF) and, using this mAb, tissue distribution of guinea‐pig DAF was studied by immunofluorescence. Guinea‐pig DAF was found to be expressed not only on the vascular endothelium but also on different types of cells, such as the tubular epithelium of the kidney, epidermal cells of the skin and synovial lining cells. As there was no significant reduction in staining intensity with MCA44 following treatment with phosphatidylinositol‐specific phospholipase C, many guinea‐pig DAF molecules expressed in these tissues may be of the transmembrane form.