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Interactions of dendritic cells with fibronectin and endothelial cells
Author(s) -
Carolina Jancic,
Eduardo Chuluyan,
Adrián E. Morelli,
Adriana T. Larregina,
E. Kolkowski,
Mónica Saracco,
Marcos Barboza,
W S Leiva,
Leonardo Fainboim
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00586.x
Subject(s) - cd86 , integrin , fibronectin , dendritic cell , microbiology and biotechnology , immunology , chemistry , t cell , biology , antigen , cell , biochemistry , extracellular matrix , immune system
We studied the phenotypic characteristics of spontaneously migrated skin dendritic cells (sDC) and monocyte‐derived dendritic cells (moDC), generated under different culture conditions, and their interactions with fibronectin (FN) and endothelial cells. Monocyte‐derived dendritic cells were obtained after culturing monocytes with granulocyte–macrophage colony‐stimulating factor (GM‐CSF) (800 U/ml) and interleukin‐4 (IL‐4) (500 U/ml) with either 10% fetal bovine serum (FBS) or 10% allogeneic human serum (HS). Regardless of the type of serum used, the majority of moDC expressed human leucocyte antigen‐DR (HLA‐DR) and CD86. On day 5 of incubation, 20–67% of moDC cultured in the presence of HS (HS‐moDC) expressed CD1a, b and c versus 94–97% when cultured in the presence of FBS (FBS‐moDC). DC showed a differential gradient of adhesion to FN: FBS‐moDC>HS‐moDC>sDC≈monocytes. Both FBS‐moDC and HS‐moDC were strongly positive for CD49e (α5‐integrin) and CD29 (β1‐integrin) but negative for CD49d (α4‐integrin). A monoclonal antibody (mAb) against CD49e blocked the adhesion of both types of moDC to FN. Although both FBS‐moDC and HS‐moDC attached to endothelium (a 76% and 63% increase, respectively), only HS‐moDC were able to migrate through non‐activated endothelium. Overall, these results suggest that spontaneously migrated sDC are less adherent to FN than moDC, that HS and FBS induce differences in CD1 expression, that HS‐moDC are less adhesive to FN and endothelial cells but more motile than FBS‐moDC, and that α5β1‐integrin is the molecule involved in moDC adhesion to FN.