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Fas ligand (FasL, CD95L, APO‐1L) expression in murine mast cells
Author(s) -
Wagelie-Steffen Al,
Karin Hartmann,
H. Vliagoftis,
D D Metcalfe
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00559.x
Subject(s) - fas ligand , jurkat cells , mast cell , microbiology and biotechnology , biology , apoptosis , flow cytometry , tumor necrosis factor alpha , immunology , t cell , programmed cell death , immune system , biochemistry
Fas ligand (FasL, CD95L, Apo‐1L), a type II membrane protein belonging to the tumour necrosis factor family, induces apoptosis in Fas‐bearing cells. As murine mast cells have been shown to express Fas antigen, we hypothesized that mast cells might also express FasL. To explore this possibility, we first demonstrated FasL mRNA in mast cells by reverse transcription–polymerase chain reaction and FasL protein by immunoblot analysis. FasL protein was shown to be exclusively located within the cell by flow cytometry. In agreement with this observation, bone marrow cultured mast cells were unable to kill Jurkat T cells. Our results demonstrate that FasL is expressed in murine mast cells and suggest that this murine mast cell FasL is not lytic, owing to the intracellular localization.