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Immuno‐stimulatory effects of bacterial‐derived plasmids depend on the nature of the antigen in intramuscular DNA inoculations
Author(s) -
LEE S. W.,
SUNG Y. C.
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00557.x
Subject(s) - plasmid , biology , ctl* , dna vaccination , antigen , immune system , cytotoxic t cell , dna , virology , microbiology and biotechnology , nucleoprotein , virus , immunology , cd8 , in vitro , genetics
The CpG motifs of bacterial‐derived plasmids augment antigen‐specific immune responses and steer those responses towards the T helper 1 (Th1) type. In this study, we have addressed the immuno‐stimulatory effect of intramuscular co‐administration of CpG motifs containing vector DNA on the modulation of immune responses to the haemagglutinin (HA) and the nucleoprotein (NP) proteins of influenza virus. The co‐administration of vector DNA with a HA‐encoding plasmid DNA showed a significant enhancement in the total IgG response, the generation of cytotoxic T lymphocyte (CTL), and the T‐cell proliferative response. In the case of NP‐encoding plasmid DNA inoculations, the co‐administration of vector DNA slightly decreased the total IgG response, although the IgG2a/IgG1 ratio and the CTL responses to NP were significantly increased. These observations suggest that the immuno‐stimulatory effects of bacterial‐derived plasmids depend upon the nature of the co‐administered antigen.